GuitarBrdArounO's movie Reviews on Newgrounds

[A Stick Tagedy]

good job

you definatly have potential, just touch up some stuff, maybe fix the clouds. but anyway, your backrounds are exellent, try to make actual characters, rather then sticks, nobody wants to look at sticks all day, sticks are way overused, so please just take a little more time and youll get it! i gave you a 3 or a 2 a cant remember

[Reh-tarded]

it was a nice flash

good job, but your an idiot! THAT SONG WAS ORIGINALLY DONE BY PINK FLOYD! use his, its better . lol!
MARILYN MANSON SUX NUT SAX!
anyway good job, quite random, i like random

Killjoy-6969 responds:

1st of all...the original didnt fit it properly. i do agree that it is a better song, but i chose this because its a little edgyer...and no...he doesnt suk nut sax...hes very talented...just very very VERY fuked up. i like his musik just not him...

[the music is cool right 4]

nice

your awsome at drawing, keep it up!

[Merry Melony ep.1]

Very good

It's really good, dont get me wrong but i dont think it should be the greatest of all time, dont take that offensive, but nice job

[Eye Robot]

AWSOME DUDELY!

It was pretty lame but i really like the fact that the Insects encounter several microbes during different stages of growth and development. Incidental entry of these microbes elicits a host defense response, which mainly consists of aphenol oxidase pathway. The invasion of the microbe is recognised by a specific protein which activates the prophenol oxidase activating enzyme (PPAE). The activated PPAE in turn switches "on" the phenol oxidase, which leads to encapsulation of the invading organism. Understanding the mechanisms of insect immunity will allow us to discover potential targets for inactivating the mechanism of immune action in insects and make them vulnerable to natural microbial infection. We have identified PPAE and phenol oxidase encoding genes from major agricultural pests, H. armigera and Spodoptera litura. A database search for homology to other phenol oxidase of other pests classified S. litura enzymes as phenol oxidase class II. The transcription of gene is induced upon microbial challenge of sixth instar larvae with E. coli and is unresponsive to wound injury. The gene has been cloned and expressed in an E. coli expression vector, pET43. Induction with IPTG resulted in its accumulation into a soluble fraction of cells. The expressed enzyme is catalytically active on dopamine upon activation by cetyl pyridinium chloride. The regulatory role played by this enzyme in insect immunity is being examined.

Insecticidal proteins from Xenorhabdus and Photorhabdus

We have been working on the identification of alternate molecules produced by soil organisms like Xenorhabdus and Photorhabdus with insecticidal activity to enhance the action of Bt toxins and study their mode of action to plan strategies for resistance management in the insects. Xenorhabdus nematophilus is a Gram-negative bacterium belonging to the family enterobacteriaceae. It lives in symbiotic association with entomopathogenic nematodes of the family Steinernematidae. X. nematophilus is pathogenic to a wide range of insects and is currently being used as a biological control agent against Lepidoptera, Coleoptera and Diptera as spray formulations. We have been investigating the insecticidal potential of the proteins secreted by X. nematophilus in the culture medium. We have isolated large protein complexes from the culture medium with oral larvicidal activity. These outer membrane vesicle (OMV) associated proteins, when incorporated in the diet, were found to be active against common lepidopteran pests Helicoverpa armigera and Spodoptera litura. Some of the individual proteins present in the large complex have been characterised. Strong chitinase activity and bacteriocin-like proteins were found to be associated with the protein complex. A 17 kDa pilin protein was isolated, purified and cloned. Pilin protein constitutes hair-like appendages on the surface of a bacterial cell, that mediate host cell recognition and adherence. Interestingly, the evaluation of biological activity of the pilin protein from X. nematophilus, revealed their larvicidal nature for common agricultural pests.

Comparison of the native pilin isolated from the bacteria with the recombinant protein showed that oligomerisation of the protein is critical for larvicidal activity. To understand the basis of oligomerisation, we produced point mutations in the N-terminal region of the pilin gene, and analysed the behaviour of the mutants. Three conserved hydrophobic residues in the N-terminus of the protein were altered individually and together to yield four mutant polypeptides. Comparison of the far UV CD spectrum of native protein with the recombinant protein showed the presence of a negative peak at 215 nm characteristic of an ordered structure comprised of <-sheets, while the CD spectra of the mutant proteins displayed a progressive decrease in the peak intensity

[Robot Raptor]

the graphics were nice.

I liked it but it had some negetive comeback on my part, Ive studied this my whole life and this is what I came up with....During growth, insects go through repeated cycles of moulting necessitating dissolution of chitin to accommodate an enlarging body. Therefore, expression of chitinase in insect larvae coincides with the moulting process and is developmentally controlled by hormones. Since chitin is a structural component in insects, its synthesis and hydrolysis are considered to be potential targets for pest management. A detailed analysis of chitin dissolution was undertaken to evaluate it as a target for a chitinase-based pesticide. We have earlier described cloning of chitinase from Helicoverpa armigera. The Helicoverpa chitinase cDNA is 2870 bp in length and contains an open reading frame of 1767 bp. The cDNA has a GC rich 5' untranslated region of 47 bp and a 3' untranslated region of 1057 bp. The sequence of translation initiation codon C A A A A T G A agrees with the Drosophila start site A A A C A T G more closely than the Kozak sequences, G C C A T G G. A polyadenylation signal sequence is present at position 2852 within the 3' UTR. The ORF encodes a polypeptide of 588 residues with a predicted molecular mass of 66 kDa. The polypeptide has two main domains, a catalytic glycosyl hydrolase conserved domain, FDGLDLDKE, and a conserved peritrophic chitin-binding domain at the C-terminus, which contained six conserved cysteines. The catalytic and chitin binding domains are held together by a proline threonine rich region which is longer in Helicoverpa than in other lepidopteran insects.

This chitinase, when expressed using a prokaryotic vector in E. coli was catalytically inefficient and hence it was cloned and expressed in Trichoplusia ni cell line using a baculovirus vector. The recombinant chitinase in T. ni cells accumulated in the culture medium and was purified by ion exchange chromatography. The purified protein displayed high catalytic activity against polymeric substrate on a glycol chitin activity gel and the fluorometric oligosacchacide substrates. The enzyme preferred trisaccharride to disaccharides on substrate and hence classified as an endochitinase, displayed bimodal pH optima at 6.0 and 7.5 and was maximally active at 45oC. Using quantitative RT-PCR chitinase transcript was found in the integument, gut, and fat bodies but was absent in haemocytes. The developmental expression of chitinase was studied in the midgut and integument by RT-PCR. The integument and midgut of larvae were collected for seven days and included moultings from fourth to fifth instar and from fifth to pupal stage. The gene-specific product was obtained at all stages under study but the relative abundance varied at each stage. A high amount of transcript was present during moulting and barely detectable levels were observed during intermoulting stages. Identical expression patterns were obtained in the integument and the midgut. Western blot analysis and integument extracts with chitinase specific antibodies showed that the enzyme was present only during moulting stages and was absent in the intermould periods. It is likely that the chitinase expression is regulated post-transcriptionally and the mRNA is translated only at the moulting stages when enzyme activity is required to degrade the cuticle

bobjarb responds:

your wierd

[teh rings]

ok... seriosly, do people want to watch spiny ring

I dont like it.... maybe if it had a plot or was funny or if the pizzas went diagonel to eatchother, but Ill i can say is good graphics but sHITTY MOVIE AND I HATE YOU! YOUR A DISGRACE TO THE ALIEN EMPERER AND THE SHINY CIRCLES OF LIFE AND DESTINY.... by the way, did you get that thing i sent you?

Are-you-feelin-ok responds:

Hey man thanks and im sorry about the alien empire thing, but yeah I've taken what you said into account, thanks for the advice..

[broyvms--random]

Good!

For the dipshit infront of me, HE WASNT TRYING TO BE FUNNY! DADS HOME HAS NO REFRENCE TO THIS MOVIE AT ALL! anyway nice job, try to make it longer next time! you got a 4

IrMyke responds:

haha thank you! YOUR RIGHT!1!!!! thanks i will make it longer , AND yeah

[The Mario: Reloaded]

Very damn good for a first submission

I liked it, some other people made mario matrix but u kept with the whole idea of mario instead of the matrix, thats what i liked the most but, keep it up and Space adventures or watever is front page worthy!

[Carl Spacedew]

All I can say is...

Front page worthy! lol holy shit that was nice
I kept waiting for one thing tho, i was waiting for some alien to get all pissed off because is ship was gone. But damn nice, keep up the great work

Falconer02 responds:

Thanks! Will do!